Infrared spectrophotometry of enteric bacteria.

نویسندگان

S LEVINE

H J R STEVENSON

L A CHAMBERS

B A KENNER

چکیده

Infrared spectrophotometry is rapidly assuming an important place in the biological sciences. Its value derives from the fact that each organic compound has a characteristic and distinct infrared spectrum that can be used to identify it (Randall et al., 1949). The biologist working with cells or tissues is confronted by a highly complex mixture of substances. The infrared spectrum of a cell, therefore, represents only an over-all chemical composition. It cannot, because of the complexity of the mixture, be interpreted quantitatively in terms of individual compounds. On the other hand, this method is simple, rapid, and versatile. It has been applied to tissues and cells without preliminary treatment other than drying by Barer, Cole, and Thompson (1949), Blout and Mellors (1949), and Schwarz et al. (1951). Randall, Smith, Colm, and Nungester (1951) have demonstrated the reproducibility of infrared spectra of chloroform extracts of tubercle bacilli and have indicated their usefulness in the control and development of fractionation procedures. Whole bacterial cells have been examined by Stevenson and Bolduan (1952) who showed that many species of bacteria were sufficiently varied in chemical composition to be differentiated by their infrared spectra. This paper represents a continuation of their work with emphasis on enteric bacteria.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Glycogen of enteric bacteria.

In the course of an investigation of the use of infrared spectrophotometry for bacterial identification (Stevenson and Bolduan, 1952; Levine et al., 1953b), it has been found that a characteristic set of absorption bands appears in the spectra of Enterobacteriaceae and other bacteria under certain cultural conditions. Since this set of bands is common to many organisms, it is important in ident...

متن کامل

Synthesis of Some Novel 2-Amino-5-arylazothiazole Disperse Dyes for Dyeing Polyester Fabrics and Their Antimicrobial Activity.

The present work describes the synthesis of a series of four novel biologically active 2-amino-5-arylazothiazole disperse dyes containing the sulfa drug nucleus. The structures of the synthesized thiazole derivatives are confirmed using UV-spectrophotometry, infrared and nuclear magnetic resonance techniques and elemental analysis. The synthesized dyes are applied to polyester fabrics as disper...

متن کامل

Design of a Near Infrared Spectrophotometry Brain Imaging System

Measuring brain activity is at task generally left for EEG or fMRI. However, there is a lesser known imaging technique known as near infrared spectrophotometry, in which near infrared light is shown into the patients head to measure changes in oxygenation that directly relate to brain activity. This paper describes a low-cost, wireless, portable near infrared spectrophotometry system that is ab...

متن کامل